Engineering novel optical approaches
We build high-speed microscopes to image action potentials across large populations of neurons expressing genetically encoded voltage sensors. To selectively activate or inhibit small sets of cells, we utilize holographic optogenetics.
▷ in vivo voltage imaging of molecular layer interneurons at 5,000 fps
Linking neural activity to behavior
Our toolkit enables in vivo voltage imaging from genetically defined neural populations in the cerebellum as well as optogenetic tests of their functional role in mice engaged in sensorimotor tasks. We apply machine learning to the rich datasets from our experiments to reveal how specific cell types contribute to communication with the sensory periphery and muscles.
▷ mouse whisker movements imaged at high-speed
Decoding layer-specific computation
The cerebellum is organized into thin laminae composed of cell types that serve different yet complementary roles. We study how the functional connectivity and firing properties of these layered circuits give rise to emergent computations critical for cognition and the control of movement.
▷ in vivo z-stack of Golgi cells in the cerebellar granular layer